Neuronal density distributions are assessed by dividing the evaluated migration area (Migration distance) into ten rings (a+b) with equal widths. In each ring the ratio between the number of neurons and the number of cell nuclei is normalized to the ratio within the entire migration area. This results in a distance dependent function of neuronal density. Omnisphero fascilitates the possibility to import output of other image analysis software for reevaluation and comparison with other image analysis methods. Additionally, also density distributions of other cell types can be evaluated if coordinates of those are available.