Density distributions

Neuronal density distributions are assessed by dividing the evaluated migration area (Migration distance) into ten rings (a+b) with equal widths. In each ring the ratio between the number of neurons and the number of cell nuclei is normalized to the ratio within the entire migration area. This results in a distance dependent function of neuronal density. Omnisphero fascilitates the possibility to import output of other image analysis software for reevaluation and comparison with other image analysis methods. Additionally, also density distributions of other cell types can be evaluated if coordinates of those are available.

Density distributions
Neuronal density distribution: Point clouds of the centroids of identified nuclei (green) and neurons (magenta) (a) as well as the original image (b) are overlapped with the mask obtained by the assessment of the radial migration which is subdivided into ten rings with equal width . For each ring the ratio between neurons (magenta) and cell nuclei (green) is evaluated and normalized to the average ratio within the entire migration area. This results in a distance dependent density distribution of neurons.