Radial migration distance is defined as the average distance between the rim of the sphere-core and the furthest migrated cells. Thus migration distance is assessed by recognizing the sphere-core and defining it as the inner border of the migration area. The outer border is identified by using the density of coordinates of the identified centroids of cell nuclei and the intensity information of the nucleus channel. The migration distance is subsequently calculated as the mean distance between these two borders. Since only the raw images and the centroids of cell nuclei are required to assess this endpoint, centroids of cell nuclei obtained by different methods can be imported in Omnisphero and used for the evaluation.